The Shape, Size and Uniformity Control of Biodegradable Poly(lactic-co-glycolic acid) Micro-Particles Produced by Electrospraying Method

Document Type : Research Paper

Author

Department of Biological Systems, Faculty of New Technologies Engineering, Shahid Beheshti University, Postal Code 1983969411, Tehran, Iran

Abstract

Hypothesis: Nowadays, the growing concern over environmental pollution has attracted attentions towards development of biodegradable micro-particles to replace non-degradable micro-particles used in cosmetic products. In this regard, poly(lactic-co-glycolic acid) (PLGA) micro-particles produced by a simple technique, electrospraying, is used as biodegradable micro-particles. To obtain the maximum efficiency, especially as a drug delivery system, these particles need to be fiber-free with spherical shape and have a uniform size distribution. This goal can be achieved by controlling variables such as polymer molecular weight, solvent type, polymer solution concentration, voltage, needle size, polymer solution feed rate and distance from needle tip to collector.
Methods: PLGA particles were electrosprayed considering four variables, including polymer solution concentration (at three levels), needle size (at two levels), voltage (at three levels), and polymer solution feed rate (at three levels). Then, electron scanning microscopic images were obtained from each sample to observe the morphology of particles and to study the effect of process variables on it. To understand the morphological behavior of the particles, the influence of each variable was investigated according to mechanisms involved in electrospraying process.
Findings: The four variables affecting droplet size, and consequently, Peclet number and droplet charge play important roles in mechanisms involved in electrospraying process and determining the morphology of the resultant particles. Thanks to the effects of these phenomena, spherical and fiber-free micro-particles with uniform size were obtained by electrospraying PLGA solutions at concentrations of 3% and 5%, using a 27 G needle at a feed rate of 0.3 mL/h and applying a voltage of 10 kV.

Keywords

References

  1. Nam H. C.and Park, W.H., Eco-friendly Poly(lactic acid) Microbeads for Cosmetics via Melt Electrospraying, Int. J. Biol. Macromol., 157, 734-742, 2020.
  2. Kapoor D.N., Bhatia A., Kaur R., Sharma R., Kaur G., and Dhawan S., PLGA: A Unique Polymer for Drug Delivery, Ther. Deliv., 6, 41-5, 2015.
  3. Mir M., Ahmed N., and ur Rehman, A., Recent Applications of PLGA Based Nanostructures in Drug Delivery, Colloids Surf. B Biointerfaces, 159, 217-231, 2017.
  4. Ito F., Takahashi T., Kanamura K., and Kawakami H. Possibility for the Development of Cosmetics with PLGA Nanospheres, Drug Dev. Ind. Pharm.,39, 752-761, 2013.
  5. Vatankhah E., Prabhakaran M.P., and Ramakrishna S., Stem-Cell Nanoengineering, Wiley-Blackwell,123-141, 2015.
  6. Bock N., Woodruff M.A., Hutmacher D.W., and Dargaville T.R., Electrospraying, a Reproducible Method for Production of Polymeric Microspheres for Biomedical Applications. Polymers, 3, 131-149, 2011.
  7. Verreck G., Chun I., Peeters J., Rosenblatt J., and Brewster M.E., Preparation and Characterization of Nanofibers Containing Amorphous Drug Dispersions Generated by Electrostatic Spinning, Pharml. Res., 20, 810-817, 2003.
  8. Verreck G., Chun I., Rosenblatt J., Peeters J., Van Dijck A., Mensch J., Noppe M., and Brewster M. E., Incorporation of Drugs in an Amorphous State into Electrospun Nanofibers Composed of a Water-insoluble, Nonbiodegradable Polymer, J. Control. Release, 92, 349-360, 2003.
  9. Shi Y., Wei Z., Zhao H., Liu T., Dong A., and Zhang J., Electrospinning of Ibuprofen-loaded Composite Nanofibers for Improving the Performances of Transdermal Patches, J. Nanosci. Nanotechno.,13, 3855-3863, 2013.
  10. Zamani M., Prabhakaran M.P., and Ramakrishna S., Advances in Drug Delivery via Electrospun and Electrosprayed Nanomaterials, Int. J. Nanomedicine, 8, 2997-3017, 2013.
  11. Wang J., Helder L., Shao J, Jansen J. A., Yang M., and Yang F., Encapsulation and Release of Doxycycline from Electrospray-Generated PLGA Microspheres: Effect of Polymer End Group, Int. J. Pharm., 546, 1-9, 2019.
  12. Kibler E., Lavrinenko A., Kolesnik I., Stankevich K., Bolbasov E., Kudryavtseva V., Leonov A., Schepetkin I., Khlebnikov A., Mark T.Q., and Sergei T., Electrosprayed Poly(lactic-co-glycolic acid) Particles as a Promising Drug Delivery System for the Novel JNK Inhibitor IQ-1, Eur. Polym. J., 127, 109598, 2020.
  13. Vatankhah E., Hamedi S., and Ramezani O., Surfactant-Assisted Incorporation of Rosmarinic Acid into Electrosprayed Poly (lactic-co-glycolic acid) Microparticles with Potential for Cosmetic and Pharmaceutical Applications, Polym. Test., 81, 106180, 2020.
  14. Si S., Li H., and Han X., Sustained Release Olmesartan Medoxomil Loaded PLGA Nanoparticles with Improved Oral Bioavailability to Treat Hypertension, J. Drug Deliv. Sci. Tec., 55, 101422, 2020.
  15. Hsu M.Y., Huang Y.T., Weng C.J., Chen C.-M., Su Y.F., Chu S.Y., Tseng J.H., Wu R.C. and Liu S.J., Preparation and In Vitro/In Vivo Evaluation of Doxorubicin-Loaded Poly(lactic-co-glycol acid) Microspheres Using Electrospray Method for Sustained Drug Delivery and Potential Intratumoral Injection, Colloids Surface B, 190,110937, 2020.
  16. Yao J., Lim L.K., Xie J., Hua J.,and Wang C.H., Characterization of Electrospraying Process for Polymeric Particle Fabrication, J. Aerosol. Sci., 39, 987-1002, 2008.
  17. Almería B., Deng W., Fahmy T.M., and Gomez A., Controlling the Morphology of Electrospray-generated PLGA Microparticles for Drug Delivery, J. Colloid. Interface Sci., 343, 125-133, 2010.
  18. Nguyen D.N., Clasen C., and Van den Mooter G., Pharmaceutical Applications of Electrospraying, J. Pharm. Sci., 105, 2601-2620, 2016.
  19. Hsu M.Y., Feng C.H., Liu Y.W., and Liu S.J., An Orthogonal Model to Study the Effect of Electrospraying Parameters on the Morphology of Poly(d,l)-lactide-co-glycolide (PLGA) Particles, Appl. Sci., 9, 1077, 2019.
  20. Morais A.Í., Vieira E.G., Afewerki S., Sousa R.B., Honorio L., Cambrussi A.N.C.O., Santos J.A., Bezerra R.D.S., Furtini J.A.O., and Silva-Filho E.C., Fabrication of Polymeric Microparticles by Electrospray: The Impact of Experimental Parameters, J. Funct. Biomater., 11, 4, 2020.
  21. Xie J., Lim L.K., Phua Y., Hua J., and Wang C.H., Electrohydrodynamic Atomization for Biodegradable Polymeric Particle Production, J. Colloid Interf. Sci., 302, 103-112, 2006.
  22. Prabhakaran M.P., Zamani M., Felice B., and Ramakrishna S., Electrospraying Technique for the Fabrication of Metronidazole Contained PLGA Particles and Their Release Profile, Mater. Sci. Eng., C: Mater. Biol. Appl., 56, 66-73, 2015.
  23. Nath S.D., Son S., Sadiasa A., Min Y.K., and Lee B.T., Preparation and Characterization of PLGA Microspheres by the Electrospraying Method for Delivering Simvastatin for Bone Regeneration, Int. J. Pharm.,443, 87-94, 2013.

 

Iranian Journal of Polymer Science and Technology
Volume 33, Issue 3 - Serial Number 167
September and October 2020
Pages 229-242

Files

Share

How to cite

Statistics