Studies on Synergistic Effect of Curcumin, Piperine and Ellagic Acid on Antibacterial Properties of Biocompatible Nanofibers Based on Polycaprolactone

Document Type : Research Paper

Authors

1 Department of Chemical and Polymer Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

2 Biomaterials Department, Iran Polymer and Petrochemical Institute, P.O. Box: 14975-112, Tehran, Iran.

Abstract

Hypothesis: The fabrication of electrospun nanofibers for drug delivery applications has attracted a great attention in recent years. Polycaprolactone (PCL) is a polymer which has been used frequently in medical and tissue engineering applications. However, the hydrophobic nature of PCL is a drawback which could be resolved by biological materials. The synergistic effect of piperine and ellagic acid on different properties and performance of curcumin containing nanofiber mats has been investigated.
Methods: Nanofibers based on PCL with certain amounts of plant extracts were prepared and produced through electrospinning. In order to investigate the effect of each plant extract, single systems containing curcumin or piperine, dual systems containing curcumin/piperine and triple systems containing curcumin/piperine/ellagic acid were produced by electrospinning and various analyses were performed to find out whether they were effective in improving the properties of fiber prepared for medical applications.
Findings: The study on microstructure of electrospun mats showed that the diameter distribution of nanofibers was between 50-100 nm and the average fiber diameter in single, double and triple samples changed in the range of 73-87 nm. The antibacterial test revealed that piperine is also an active antibacterial agent like curcumin. In addition, the triple system had a good degree of antibacterial activity (79%). The water uptake and water vapor permeability (WVP) of triple samples were about 337% and 11.56 mg(cm2.h)-1, respectively. The prepared mats showed desirable tensile strength, elasticity and flexibility. Cell viability analysis on electrospun mats indicated that they are not toxic to human dermal fibroblasts (HDF). Therefore, taking into account all the results, it can be concluded that the use of two compounds, ellagic acid and piperine, is effective in increasing the bioavailability of curcumin, and the mats can be used in medical applications as wound dressings..

Keywords

References

  1. Dash T.K. and Konkimalla V.B., Polymeric Modification and Its Implication in Drug Delivery: Poly(ε-caprolactone) (PCL) as a Model Polymer, Pharm., 9, 2365-2379, 2012.
  2. Zahedi E., Esmaeili A., and Eslahi N., Fabrication and Characterization of Core-Shell Electrospun Fibrous Mats Containing Medicinal Herbs for Wound Healing and Skin Tissue Engineering, Drugs.,17, 1-13, 2019.
  3. Kazemi S., Pourmadadi M., Yazdian F., and Ghadami A., The Synthesis and Characterization of Targeted Delivery Curcumin Using Chitosan-Magnetite-Reduced Graphene Oxide as Nano-carrier, J. Biol. Macromol., 186, 554-562, 2021.
  4. Prasad S., Tyagi A.K., and Aggarwal B.B., Recent Developments in Delivery, Bioavailability, Absorption and Metabolism of Curcumin: the Golden Pigment from Golden Spice, Cancer Res. Treat Off J. Korean Cancer Assoc., 46, 2-18, 2014.
  5. Gorgani L., Mohammadi M., Najafpour G.D., and Nikzad M., Piperine-the Bioactive Compound of Black Pepper: from Isolation to Medicinal Formulations, Rev. Food Sci. Food Saf., 16,124-140, 2017.
  6. Scrimshaw N.S., Handbook of Herbs and Spices, Book Review, 3, 249-249, 2007.
  7. Bang J.S., Choi H.M., Sur B.J., and Lim S.L., Anti-inflammatory and Antiarthritic Effects of Piperine in Human Interleukin 1β-Stimulated Fibroblast-Like Synoviocytes and in Rat Arthritis Models, Arthritis Res. Ther., 11, 1-9, 2009.
  8. Mohanraj V.J. and Chen Y., Nanoparticles-A Review, Trop. J. Pharm. Res., 5, 561-573, 2006.
  9. Maherani B., Arab-Tehrany E., and Mozafari M., Liposomes: A Review of Manufacturing Techniques and Targeting Strategies, Curr. Nanosci.,7, 436-452, 2011.
  10. Pentak D., In Vitro Spectroscopic Study of Piperine-Encapsulated Nanosize Liposomes, Biophys. J., 45, 175-186, 2016.
  11. Shaik M.M. and Kowshik M., Ellagic Acid Containing Collagen-Chitosan Scaffolds as Potential Antioxidative Bio-materials for Tissue Engineering Applications, Int. J. Polym. Mater. Polym. Biomater., 68, 208-215, 2019.
  12. Desai P., Thumma N.J., and Wagh P.R., Cancer Chemoprevention Using Nanotechnology-Based Approaches, Pharmacol., 11,1-9, 2020.
  13. Jantarat C., Sirathanarun P., and Boonmee S., Effect of Piperine on Skin Permeation of Curcumin from a Bacterially Derived Cellulose-Composite Double-Layer Membrane for Transdermal Curcumin Delivery, Sci. Pharm., 86, 1-14, 2018.
  14. Baspinar Y., Üstündas M., Bayraktar O., and Sezgin C., Curcumin and Piperine Loaded Zein-Chitosan Nanoparticles: Development and In-Vitro Characterisation, Saudi. Pharm. J., 26, 323-334, 2018.
  15. Saeed S.M., Mirzadeh H., Zandi M., and Barzin J., Designing and Fabrication of Curcumin Loaded PCL/PVA Multi-Layer Nanofibrous Electrospun Structures as Active Wound Dressing, Prog. Biomater., 6, 39-48, 2017.
  16. Alexandre E.M.C., Silva S., and Santos S.A.O., Antimicrobial Activity of Pomegranate Peel Extracts Performed by High Pressure and Enzymatic Assisted Extraction, Food Res. Int., 115, 167-176, 2019.
  17. Fernández J., Ruiz-Ruiz M., and Sarasua J.R., Electrospun Fibers of Polyester, with Both Nano- and Micron Diameters, Loaded with Antioxidant for Application as Wound Dressing or Tissue Engineered Scaffolds, ACS Appl. Polym. , 1,1096-1106, 2019.
  18. Liu J., Xu L., and Liu C., Preparation and Characterization of Cationic Curcumin Nanoparticles for Improvement of Cellular Uptake, Polym., 90, 16-22, 2012.
  19. Malikmammadov E., Tanir T.E., and Kiziltay A., PCL and PCL-Based Materials in Biomedical Applications, Biomater. Sci. Polym. Ed., 29, 863-893, 2018.
  20. Marković Z., Kováčová M., and Mičušík M., Structural, Mechanical, and Antibacterial Features of Curcumin/Polyurethane Nanocomposites, Appl. Polym. Sc., 136, 47283-47291, 2019.
  21. Gupta A., Gupta M., and Gupta S., Isolation of Piperine and Few Sesquiterpenes from the Cold Petroleum Ether Extract of Piper Nigrum (Black Pepper) and Its Antibacterial Activity, J. Pharmacogn. Phytochem. Res., 5, 3-7, 2013.
  22. Jaisinghani R.N., Makhwana S., and Kanojia A., Study on Antibacterial and Flavonoid Content of Ethanolic Extract of Punica Granatum (Pomegranate) Peel, Res., 9, 6-9, 2018.
  23. Rosas-Burgos E.C., Burgos-Hernández A., and Noguera-Artiaga L., Antimicrobial Activity of Pomegranate Peel Extracts as Affected by Cultivar, Sci. Food. Agric., 97, 802-810, 2017.
  24. Ranjbar-Mohammadi M. and Bahrami S., Electrospun Curcumin Loaded Poly(ε-caprolactone)/Gum Tragacanth Nanofibers for Biomedical Application, Int. J. Biol. Macromol., 84, 448-456, 2016.
  25. Wong S.C., Baji A., and Leng S., Effect of Fiber Diameter on Tensile Properties of Electrospun Poly(ɛ-caprolactone), Polymer, 49, 4713-4722, 
  26. Mutlu G., Calamak S., Ulubayram K., and Guven E., Curcumin-Loaded Electrospun PHBV Nanofibers as Potential Wound-Dressing Material, J. Drug. Deliv. Sci. Technol., 43, 185-193, 
  27. Zahedi P., Rezaeian I., and Ranaei-Siadat S., A Review on Wound Dressings with an Emphasis on Electrospun Nanofibrous Polymeric Bandages, Adv. Technol., 21,77-95, 2010.
  28. Jain S., Meka S.R.K., and Chatterjee K., Engineering a Piperine Eluting Nanofibrous Patch for Cancer Treatment, ACS Biomater. Sci. Eng., 2, 1376-1385, 2016.
  29. Sedghi R., Sayyari N., and Shaabani A., Novel Biocompatible Zinc-Curcumin Loaded Coaxial Nanofibers for Bone Tissue Engineering Application, Polymer, 142, 244-255, 2018.
Iranian Journal of Polymer Science and Technology
Volume 34, Issue 5 - Serial Number 175
January and February 2022
Pages 457-468

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