Determination of Glucose with Amperometric Method Using Polyaniline/Glutaraldehyde/Glucose Oxidase Modified Electrode

Document Type : Research Paper

Authors

Iran Polymer and Petrochemical Institute

Abstract

The polyaniline-glucose oxidase (PANI-GOD) electrode has been investigated in the present work. Polyaniline (PANI) film via cyclic voltammetric method was synthesized at room temperature, in a standard three-electrode cell. Aniline films were deposited from 1 M acidic (H2SO4) aqueous media containing
0.2 M aniline by voltammetric sweep between −0.1 V and 1 V, Ag/AgCl, at 50 mV/s. The sweep was stopped after 30 cycles at −0.1 V Ag/AgCl and the working electrode was deposited by polyaniline. Platinum plate (0.4cm×0.4cm), platinum rod and Ag/AgCl electrodes were used as working, counter and reference electrodes, respectively. The synthesized PANI films were characterized by electrochemical technique, electrical conductivity, UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS). A simple technique was used for fabrication of a glucose sensor. In this method glucose oxidase as an enzyme was immobilized by glutaraldehyde 0.1% as cross-linking agent and kept pH around 7 using 0.1 M phosphate over polyaniline thin films, glucose oxidase
was deposited on a platinum plate in phosphate or acetate buffer. The results of EIS indicated the successful immobilization of enzyme over polymer films. Effects of some experimental variables such as cross-linking agent volume content, pH, applied potential, and temperature on the amperometric response of the sensor were investigated. It was obsereved that the activity of modified electrodes changed with various amounts of cross-linking agent volume percent. The activation energy for polyaniline-modified electrode in acetate and phosphate buffer were obtained as 41 and 37 kJ/mol, respectively. A maximum current response was resulted at pH 7 and potential 0.65 V (versus Ag/AgCl).

Keywords

Iranian Journal of Polymer Science and Technology
Volume 25, Issue 6 - Serial Number 6
March and April 2013
Pages 421-431

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