Studying the Physicochemical and Biological Properties of Hydrogels Based on Laponite and Modified Chitosan through the Substitution of the Amino Group

Document Type : Research Paper

Authors

1 Student, Amirkabir university

2 Amirkabir university

3 IPPI

4 Assoc. prof., Iran Polymer and Petrochemical Institute

Abstract

Hypothesis: This study was conducted with the aim of synthesizing and identifying an injectable hydrogel based on chitosan/laponite. First, water-soluble chitosan was obtained from the reaction of chitosan/glycidyl trimethylammonium chloride (GTMAC). Then, an injectable hydrogel was prepared from its combination with Laponite nanoparticles.
Methods: Chitosan was reacted with GTMAC with ratios of 1:1, 3:1 and 6:1. Using FTIR and H1NMR, the successful synthesis of water-soluble chitosan was confirmed. Besides that; Thermal stability, solubility in water, ability to absorb and retain moisture, zeta potential, biocompatibility and antibacterial activity of modified samples were investigated and compared with pure chitosan. Then, the optimal sample of modified chitosan was combined with laponite at a ratio of 1:1, and the physicochemical, injectability, self-healing, rheological, and biocompatibility properties of this hydrogel were investigated.
Findings: The substitution percentage of QCS1, QCS3 and QCS6 samples was above 25%, 50% and 74%, respectively. QCS3 and QCS6 samples showed better water solubility at different pHs. By increasing the percentage of substitution, the surface charge became more positive and the antibacterial activity was improved. However, the higher percentage of cell viability in the QCS3 sample makes it more suitable for biological applications. After hydrogel formation, the ionic and hydrogen interactions between the QCS/LAP in the hydrogel was confirmed by FTIR. Elemental analysis confirmed the uniform distribution of laponite in the hydrogel. SEM images showed a reduction in pore size after incorporating Laponite in the hydrogel. Rheological studies showed a 5-fold increase in the mixed shear modulus after the addition of laponite. Also, the gelation time of the hydrogel was calculated about 5 minutes. The percentage of cell viability obtained by the MTT test after 72 hours was 93%. Consequently, the introduced hybrid hydrogel can be a suitable choice for tissue engineering and drug delivery applications.

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