نانوالیاف هسته-پوسته فیبروئین ابریشم-پلی‌وینیل الکل: ساختار و رفتار رهایش کنترل شده

نوع مقاله: پژوهشی

نویسندگان

رشت، دانشگاه گیلان، دانشکده فنی، گروه مهندسی نساجی، صندوق پستی 3756

چکیده

ساختارهای نانولیفی هسته-پوسته تهیه‌ شده از پلیمرهای زیست‌سازگار و زیست‌تخریب‌پذیر نظیر فیبروئین ابریشم، کاربردهای بالقوه فراوانی در مهندسی بافت و دارورسانی دارند. در این بررسی، الکتروریسی هم‌محور فیبروئین ابریشم به‌عنوان جزء پوسته و ترکیب سالیسیلیک ‌اسید و پلی‌وینیل‌الکل در هسته، برای تهیه ساختار نانولیفی هسته-پوسته انجام شد. اثر تغییرات غلظت، رسانایی الکتریکی و گرانروی ‌محلول‌ها با استفاده از روش‌های طیف‌سنجی‌ زیرقرمز تبدیل فوریه (FTIR)، گرانروی‌سنجی و رسانایی‌سنجی محلول‌ها، طیف‌نورسنجی فرا‌بنفش، میکروسکوپی‌های الکترونی پویشی (SEM) و عبوری (TEM) بر قطر نهایی نانوالیاف هسته-پوسته تهیه ‌شده و رفتار رهایش آن‌ها بررسی شد. در نهایت سعی شد تا مدل مناسبی برای رفتار رهایش سالیسیلیک اسید از این ساختار هسته-پوسته ارائه شود. نتایج نشان داد، قطر نهایی نانوالیاف هسته-پوسته به‌دست آمده در محدوده  (22±)110nm تا nm  (71±) 250 و قطر هسته آن‌ها 40nm تا 80nm متغیر بود. با توجه به نقش رسانا‌کنندگی جزء پوسته در الکتروریسی هم‌محور، ارتباط مستقیم بین افزایش غلظت محلول پوسته و قطر نهایی در نتایج مشاهده شد، درحالی‌که افزایش غلظت محلول هسته در همه شرایط اثر معناداری بر قطر الیاف الکتروریسی ‌شده نداشت. نتایج نشان داد، غلظت محلول هسته تنها عامل تعیین‌کننده‌ قطر نانوالیاف نیست و اثر اختلاف گرانروی و رسانانندگی الکتریکی محلول ‌پوسته و هسته نیز نقش مؤثری بر قطر نهایی نانوالیاف دارد. مطالعه رهایش سالیسیلیک اسید نشان داد، نسبت ترکیب درصد پوسته و هسته، عامل مؤثری بر مقدار و سرعت رهایش از این ساختارهاست، به‌طوری که افزایش مقدار سالیسیلیک اسید رها شده و سرعت رهایش آن با افزایش درصد پلی‌وینیل‌الکل در ترکیب هسته مشاهده شد.

کلیدواژه‌ها


عنوان مقاله [English]

Core/Shell Nanofibers of Silk Fibroin/Polyvinyl Alcohol: Structure and Controlled Release Behavior

نویسندگان [English]

  • Nadia Rahimi Tanha
  • mahdi Nouri
Department of Textile Engineering, Faculty of Engineering, University of Guilan, P.O. Box: 3756, Rasht, Iran
چکیده [English]

Hypothesis: Core-shell nano-fibrous structures obtained from biodegradable and biocompatible polymers such as silk fibroin (SF) have potential applications in drug delivery and tissue engineering. In this work, coaxial electrospinning of silk fibroin as shell and salicylic acid (SA)/polyvinyl alcohol (PVA) blends as core was studied to fabricate core-shell nano-fibrous structures.
Methods: Silk fibroin was extracted from cocoon and dissolved in formic acid. An assembled coaxial nozzle was used to fabricate core-shell nanofibers of SF as shell and PVA/salicylic acid as core components, respectively.
Findings: Effects of variation in viscosity and electrical conductivity of the electrospinning solutions on the final nanofibers morphology, diameters and SA release behaviors were studied using Fourier transforms infrared spectroscopy (FTIR), viscometry, electrical conductometry, ultraviolet spectrophotometry, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. Finally, a suitable model for release behaviors of the fabricated core-shell nanofibers was suggested. It was found that the final diameter of fabricated core-shell nanofibers varied from 110 (±22) to 250 (±71) nm and diameter of the core section varied from 40 to 80 nm. The experimental results showed that the shell solution concentration had a significant effect on the final core-shell nanofiber diameter, but increase in the core solution concentration had an insignificant effect on the final nanofibers diameter. It was concluded that the core solution concentration was not the only effective parameter in determining the final diameters of electrospun nanofibers, and the viscosities of shell and core solutions and their electrical conductivities were just as important parameters. According to the results of release profiles, the ratio of the components in core and shell was the effective parameter affecting the profiles of SA released from these structures. The higher PVA concentration in the core increased both the amount of SA and its release rate from fabricated core-shell nanofibers

کلیدواژه‌ها [English]

  • coaxial electrospinning
  • core- shell
  • silk fibroin
  • controlled release
  • nanofibers
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