خواص فیزیکی-مکانیکی، رفتار رهایش و تکثیر سلولی هیدروژل پلی(وینیل الکل)-پلی(اتیلن گلیکول)-آگار دارای آلوئه‌ورا

نوع مقاله : پژوهشی

نویسندگان

1 یزد، دانشگاه آزاد اسلامی واحد یزد، دانشکده مهندسی نساجی و پلیمر، صندوق پستی 13135-89158

2 یزد، دانشگاه یزد، پردیس فنی و مهندسی،گروه مهندسی شیمی و پلیمر، صندوق پستی 741-89195

10.22063/jipst.2021.1794

چکیده

فرضیه‌: بارگذاری داروهای گیاهی در ماتریس هیدروژل‌های پلیمری، کارایی آن‌ها را برای کاربرد در زخم‌پوش به‌طور مؤثری بهبود می‌بخشد. در این راستا، مقدار داروی بارگذاری‌شده در هیدروژل نقش اساسی ایفا می‌کند و بر خواص فیزیکی-مکانیکی هیدروژل، رفتار رهایش دارو و تکثیر سلولی بر هیدروژل اثرگذار است.
روش‌ها: در این پژوهش، هیدروژل‌های بر پایه پلی(وینیل الکل)-آگار-پلی(اتیلن گلیکول) (Agar–PVA-PEG) دارای مقدارهای مختلف آلوئه‌ورا (0، 0.2، 0.5، 1 و 2wt%)  با پرتودهی الکترونی محلول آبی آن‌ها در شرایط جو محیط و دز 25kGy تهیه شدند و اثر ترکیب درصد آلوئه‌ورا بر مقدار ژل، تورم تعادلی، مقدار آب‌زدایی و خواص مکانیکی کششی هیدروژل در کنار رفتار رهایش آلوئه‌ورا از هیدروژل و رشد و تکثیر سلول‌های پوستی بر آن بررسی شد.
یافته‌ها: نتایج ارزیابی‌ها نشان داد، با افزایش مقدار آلوئه‌ورا در هیدروژل از %0.2 به %1 وزنی مقدار ژل آن از %55 به %47 کاهش  و مقدار تورم آن از %195 به %294 افزایش یافت. برخلاف انتظار، افزایش آلوئه‌ورا تا مقدار %1 وزنی به هیدروژل‌ موجب افزایش %30 استحکام کششی و افزایش %119 ازدیاد طول تا پارگی شد. پدیده‌ آب‌زدایی هیدروژل‌ در مجاورت آلوئه‌ورا تا حدی سرعت یافت. سینتیک رهایش آلوئه‌ورا از هیدروژل رفتار خطی نشان داد و بیشترین سرعت و مقدار رهایش (حدود %70) با هیدروژل دارای %1 وزنی آلوئه‌ورا به‌دست آمد. تکثیر سلول‌های فیبروپلاست نیز برای نمونه هیدروژل دارای %1 وزنی آلوئه‌ورا بیشینه بود. در نهایت، بر اساس نتایج مطالعات، مقدار بهینه آلوئه‌ورا در هیدروژل %1 وزنی تعیین شد.

کلیدواژه‌ها


عنوان مقاله [English]

Physicomechanical Properties, Release Behavior and Cell Proliferation of PVA/Agar/PEG Hydrogel Containing Aloe Vera

نویسندگان [English]

  • Hanieh , Daneshian 1
  • Mahdi Entezam 2
  • Navid Nasisrizadeh 1
1 Department of Textile and Polymer Engineering, Yazd Branch, Azad University, P.O. Box 89158-13135, Yazd, Iran
2 Department of Chemical and Polymer Engineering, Faculty of Engineering, Yazd University, P.O. Box 89195-741, Yazd, Iran
چکیده [English]

Hypothesis: Loading of herbal drug within the polymer hydrogel matrix significantly improves its performance as wound dressing. In this way, the drug amount loaded into the hydrogel plays a key role and can influence the physicomechanical properties, drug release behavior, and cell proliferation of hydrogel.
Methods: Hydrogels based on poly(vinyl alcohol) (PVA)/agar/poly(ethylene glycol) (PEG) containing various amounts (0, 0.2, 0.5, 1 and 2% by wt) of aloe vera were prepared by electron beam irradiation method at a dose of 25 kGy and keeping their water solution under the ambient atmosphere. The effect of aloe vera amount on the gel content, equilibrium swelling degree, dehydration and tensile mechanical properties of hydrogel along with aloe vera release behavior from the hydrogel and fibroblast cell proliferation was investigated.
Findings: The results obtained from the measurements indicated that with increasing aloe vera content from 0.2 wt% to 1 wt%, the gel content decreased from 55 to 47%, and the swelling degree increased from 195 to 294%. Unexpectedly, the incorporation of aloe vera to 1 wt% into the hydrogel enhanced its tensile strength by 30%, and its elongation-at-break by 119%. The dehydration phenomenon of the hydrogel was notably accelerated in the presence of aloe vera. The release kinetics of aloe vera from the hydrogel showed linear behavior and the highest release rate and amount (about 70%) was obtained for the hydrogel containing 1 wt% aloe vera. The proliferation of fibroblast cells was also the most for the hydrogel with 1 wt% aloe vera. Finally, based on the results of studies, the optimal amount of aloe vera in hydrogel was determined to be 1 wt%.


کلیدواژه‌ها [English]

  • Hydrogel
  • poly(vinyl alcohol)
  • aloe vera
  • physicomechanical properties
  • cell proliferation
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