نوع مقاله : پژوهشی
نویسندگان
1 تهران، دانشگاه تهران، دانشکدگان علوم و فناوری‌های میان رشته‌ای، دانشکده مهندسی علوم زیستی، گروه نانوبیوتکنولوژی و زیست‌تقلید، کد پستی 14399571131
2 ترکیه، آنتاکیا-هاتای، دانشگاه مصطفی کمال هاتای، دانشکده پزشکی، گروه میکروبیولوژی، کد پستی 31060
3 تهران، دانشگاه تهران، دانشکدگان علوم و فناوری‌های میان رشته‌ای،دانشکده مهندسی علوم زیستی،گروه فناوری پزشکی و مهندسی بافت، کد پستی 1439957131
4 بیرجند، دانشگاه علوم پزشکی بیرجند، دانشکده داروسازی، گروه فارماسیوتیکس و نانوفناوری، کد پستی 9717853076
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Hypothesis: The detection of pathogenic bacteria in food and clinical samples is valuable, and diagnosing them in a short period of time remains a challenge. In this research, an optical nanosensor based on Fourier transform spectroscopy from a porous silicon integrated with gelatin hydrogel was used to detect bacterial growth. Gelatin is a pH-sensitive hydrogel that swells quickly with the growth of bacteria and changes in the pH of the environment. The hybrid of this hydrogel with porous silicon nanostructure creates a pH-sensitive structure whose refractive index changes with bacterial growth and pH changes. This change causes a variation in the effective optical thickness of the hybrid nanostructure, which can be investigated using Fourier transform spectrometry
Methods: First, electrochemical etching was used to fabricate porous silicon. In the next step, the oxidized porous silicon surface was functionalized with APTES, which leaves amine groups on the surface for conjugation with gelatin .Finally, the swelling behavior of hydrogel in hybrid was investigated in response to environmental pH changes and then in the presence of different concentrations of bacteria
Findings: By X-ray energy dispersive spectroscopy in mapping mode and checking the distribution of elements, the penetration of the gel into the pores of porous silicon was observed. By analyzing the Fourier transform spectrometry of the porous siliconegelatin hybrid in the presence of buffers with different pH levels, it was determined that due to the swelling of the gel layer and the refractive index of this layer approaching the refractive index of the surrounding environment, the location of the first peak used as the fluid peak can be considered as a diagnostic point. The results showed that this structure has the ability to detect changes in environmental pH as well as bacterial growth in the concentration range of 102 to 105 cfu/mL.
کلیدواژه‌ها [English]
)